Signature Fragment Ions of Biotinylated Peptides
نویسندگان
چکیده
منابع مشابه
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Here we present a protocol to study intracellular protein-protein interactions that is based on the widely used biotin-avidin pull-down system. The modification presented includes the combination of this technique with cell-penetrating sequences. We propose to design cell-penetrating baits that can be incubated with living cells instead of cell lysates and therefore the interactions found will ...
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Protonated a(2) and a(3) (therefore doubly charged) ions in which both charges lie on the peptide backbone are formed in collision-induced dissociations of [La(III)(peptide)(CH(3)CN)(m)](3+) complexes. Abundant (a(3)+H)(2+) ions are formed from triproline (PPP) and peptides with a proline residue at the N-terminus; these peptides are the most effective in producing ions of the type (a(2)+H)(2+)...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2020
ISSN: 1044-0305,1879-1123
DOI: 10.1021/jasms.9b00024